The next year, PacBio developed a new method to allow multiple sequencing passes on the same DNA molecule. Double stranded DNA templates were ligated to two single stranded adapters, creating what the company called a “SMRTbell template.” Sequencing began at a primer on one of the adaptors and could proceed multiple times per molecule due to circularization, in a process called rolling-circle amplification. This helped reduce PacBio’s error rates significantly.
其中，约20%的HSIL可能在10年内进展为浸润性宫颈癌，但只要发现及时，并对宫颈病变，尤其是HSIL进行准确、规范治疗，就可以有效预防宫颈癌。这也是患者愿意为宫颈癌前病变付费的核心所在。
。一键获取谷歌浏览器下载是该领域的重要参考
Платон Щукин (Редактор отдела «Экономика»)
Фото: Alina Smutko / Reuters
The Stack That Should Not Exist